pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP
(Plasmid
#169333)
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PurposeExpression of HaloTag9 at the mitochondrial surface of mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169333 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDNA5/FRT/TO
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Vector typeMammalian Expression
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Selectable markersHygromycin ; EGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTOMM20-HaloTag9
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SpeciesH. sapiens (human)
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MutationHaloTag9 = HaloTag7-Q165H-P174R
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Entrez GeneTOMM20 (a.k.a. MAS20, MOM19, TOM20)
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Tag
/ Fusion Protein
- HaloTag9 (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP was a gift from Kai Johnsson (Addgene plasmid # 169333 ; http://n2t.net/addgene:169333 ; RRID:Addgene_169333) -
For your References section:
Engineered HaloTag variants for fluorescence lifetime multiplexing. Frei MS, Tarnawski M, Roberti MJ, Koch B, Hiblot J, Johnsson K. Nat Methods. 2021 Dec 16. pii: 10.1038/s41592-021-01341-x. doi: 10.1038/s41592-021-01341-x. 10.1038/s41592-021-01341-x PubMed 34916672
