pET51b(+)_HaloTag9
(Plasmid
#169324)
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PurposeExpression of HaloTag9 in bacterial cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 169324 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET51b(+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHaloTag9
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MutationHaloTag9 = HaloTag7-Q165H-P174R
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET51b(+)_HaloTag9 was a gift from Kai Johnsson (Addgene plasmid # 169324 ; http://n2t.net/addgene:169324 ; RRID:Addgene_169324) -
For your References section:
Engineered HaloTag variants for fluorescence lifetime multiplexing. Frei MS, Tarnawski M, Roberti MJ, Koch B, Hiblot J, Johnsson K. Nat Methods. 2021 Dec 16. pii: 10.1038/s41592-021-01341-x. doi: 10.1038/s41592-021-01341-x. 10.1038/s41592-021-01341-x PubMed 34916672
