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Addgene

ZP9 Actin
(Plasmid #16932)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16932 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Beta-actin
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    2500
  • Entrez Gene
    actb1 (a.k.a. ACTB, B-ACTZF, actba, bact, bactin1, bactzf, wu:fd18f05)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Antisense probe cut with EcoR1 and transcribe with T7.
Sense probe cut with Xho1 and transcribe with T3.

Plasmid History:
Constructed by Greg Kelly 5/91.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZP9 Actin was a gift from Randall Moon (Addgene plasmid # 16932 ; http://n2t.net/addgene:16932 ; RRID:Addgene_16932)
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