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SIN40C.TRE.dTomato.miR-125b-2.PGK.sfGFP.P2A.Tet3G
(Plasmid #169315)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169315 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    SIN40C
  • Vector type
    Lentiviral
  • Selectable markers
    sfGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miR-125b-2
  • Species
    H. sapiens (human)
  • Entrez Gene
    MIR125B2 (a.k.a. MIRN125B2, mir-125b-2)
  • Promoter TRE
  • Tag / Fusion Protein
    • dTomato (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SIN40C.TRE.dTomato.miR-125b-2.PGK.sfGFP.P2A.Tet3G was a gift from Jan-Henning Klusmann (Addgene plasmid # 169315 ; http://n2t.net/addgene:169315 ; RRID:Addgene_169315)

  • For your References section:

    The megakaryocytic transcription factor ARID3A suppresses leukemia pathogenesis. Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, Klusmann JH. Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231. 10.1182/blood.2021012231 PubMed 34570885
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