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Addgene

ZP11 Eng2
(Plasmid #16930)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16930 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-4
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eng2
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1800
  • Entrez Gene
    eng2a (a.k.a. eng2, ENG-2, Zf-en, ZF-EN-2, MGC193941, MGC193943)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Antisense probe: cut with EcoR1 and transcribe with T7.
Sense probe: cut with Xba1 and transcribe with SP6.

Plasmid History:
Constructed by Greg Kelly, 11/93.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZP11 Eng2 was a gift from Randall Moon (Addgene plasmid # 16930 ; http://n2t.net/addgene:16930 ; RRID:Addgene_16930)
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