SIN40C.EFS.eGFP.ARID3A-3'UTR-Mut
(Plasmid
#169296)
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PurposeConstitutive overexpression a mutated 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 169296 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSIN40C
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameARID3A-3'UTR-Mut
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SpeciesH. sapiens (human)
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MutationMutations in miR-125b-2 and let-7c binding sites
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Entrez GeneARID3A (a.k.a. BRIGHT, DRIL1, DRIL3, E2FBP1)
- Promoter EFS
Cloning Information
- Cloning method Gibson Cloning
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SIN40C.EFS.eGFP.ARID3A-3'UTR-Mut was a gift from Jan-Henning Klusmann (Addgene plasmid # 169296 ; http://n2t.net/addgene:169296 ; RRID:Addgene_169296) -
For your References section:
The megakaryocytic transcription factor ARID3A suppresses leukemia pathogenesis. Alejo-Valle O, Weigert K, Bhayadia R, Ng M, Issa H, Beyer C, Emmrich S, Schuschel K, Ihling C, Sinz A, Zimmermann M, Wickenhauser C, Flasinski M, Regenyi EM, Labuhn M, Reinhardt D, Yaspo ML, Heckl D, Klusmann JH. Blood. 2021 Sep 27. pii: S0006-4971(21)01649-9. doi: 10.1182/blood.2021012231. 10.1182/blood.2021012231 PubMed 34570885