Skip to main content
Addgene

ZP16 Wnt-1
(Plasmid #16925)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16925 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    na
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    wnt-1
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    wnt1 (a.k.a. WNT-1, int-1, sb:eu647, zgc:194464, zgc:194478)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

cDNA clone of wnt-1 for DIG labelled probes cloned by G.
Kelly. Antisense: linearize with EcoR1 and transcribe with SP6 RNA polyermase. Sense: linearize with HindIII and transcribe with T7 RNA polymerase. Hydrolyze probes for 12 minutes according to Harland, 1991.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZP16 Wnt-1 was a gift from Randall Moon (Addgene plasmid # 16925 ; http://n2t.net/addgene:16925 ; RRID:Addgene_16925)
Related items:
Commonly requested with: