pCas9-beta-mmr
(Plasmid #169241)

• Purpose: Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations
• Depositing Lab: Brian Pfleger
• Publication: Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi: 10.1016/j.ymben.2021.06.004.

Backbone

• Vector backbone: pCas9
• Vector type: Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: cas9
• Species: Streptococcus pyogenes

Gene/Insert 2

• Gene/Insert name: beta
• Species: Lambda bacteriophage

Gene/Insert 3

• Gene/Insert name: mutL-E36K
• Species: Pseudomonas putida
• Mutation: Changed glutamic acid 36 to lysine

Resource Information

• Supplemental Documents:
  • pCas9-beta-mmr.gb
• A portion of this plasmid was derived from a plasmid made by: Received plasmid with mutL mutant, pSEVA2514-rec2-mutLE36KPP, from Victor de Lorenzo from the Systems and Synthetic Biology Program, Centro Nacional de Biotecnología (CNB-CSIC).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry