eGFP-CBP
(Plasmid #169211)

• Purpose: Expression of eGFP-CBP protein for chitin visualization
• Depositing Lab: James S Fraser
• Publication: Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

Backbone

• Vector backbone: pET-28a(+)
• Backbone size w/o insert (bp): 5365
• Total vector size (bp): 6846
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Chitinase A1 Chitin Binding Domain
• Alt name: ChiA1 CBD
• Species: Bacillus circulans
• Insert Size (bp): 1554
• Promoter: T7
• Tags / Fusion Proteins:
  • eGFP (N terminal on insert)
  • Mxe Gyr Intein (N terminal on insert)
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7 fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.03.29.437438v1 for bioRxiv preprint.