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Addgene

hp-50%GC-20bp
(Plasmid #169196)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169196 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR-XL-TOPO
  • Backbone size w/o insert (bp) 3519
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    50%GC-20bp-hairpin
  • Species
    Synthetic
  • Insert Size (bp)
    5529

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer GGT AAC GCC AGG GTT TTC CCA GTC AC
  • 3′ sequencing primer GGA TAA CAA TTT CAC ACA GGA AAC AGC TAT GAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Because this sequence contains an intentional hairpin the plasmid should be cut with BstXI when sequencing this region.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hp-50%GC-20bp was a gift from Tom Perkins (Addgene plasmid # 169196 ; http://n2t.net/addgene:169196 ; RRID:Addgene_169196)

  • For your References section:

    Force-Activated DNA Substrates for In Situ Generation of ssDNA and Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay. Taylor AMK, Okoniewski SR, Uyetake L, Perkins TT. Methods Mol Biol. 2022;2478:273-312. doi: 10.1007/978-1-0716-2229-2_10. 10.1007/978-1-0716-2229-2_10 PubMed 36063324
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