pMAL513
(Plasmid
#169153)
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PurposeEncodes OptoQ-INVRT1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 169153 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVP16-EL222
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SpeciesErythrobacter litoralis
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Insert Size (bp)900
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Tag
/ Fusion Protein
- SV40 NLS (N terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byKevin Gardner and Laura Motta-Mena
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMAL513 was a gift from Jose Avalos (Addgene plasmid # 169153 ; http://n2t.net/addgene:169153 ; RRID:Addgene_169153) -
For your References section:
The Neurospora crassa Inducible Q System Enables Simultaneous Optogenetic Amplification and Inversion in Saccharomyces cerevisiae for Bidirectional Control of Gene Expression. Lalwani MA, Zhao EM, Wegner SA, Avalos JL. ACS Synth Biol. 2021 Aug 20;10(8):2060-2075. doi: 10.1021/acssynbio.1c00229. Epub 2021 Aug 4. 10.1021/acssynbio.1c00229 PubMed 34346207