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Addgene

pMAL722
(Plasmid #169149)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169149 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VP16-EL222
  • Species
    Erythrobacter litoralis
  • Insert Size (bp)
    900
  • Mutation
    A79Q
  • Tag / Fusion Protein
    • SV40 NLS (N terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Kevin Gardner and Laura Motta-Mena

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAL722 was a gift from Jose Avalos (Addgene plasmid # 169149 ; http://n2t.net/addgene:169149 ; RRID:Addgene_169149)

  • For your References section:

    The Neurospora crassa Inducible Q System Enables Simultaneous Optogenetic Amplification and Inversion in Saccharomyces cerevisiae for Bidirectional Control of Gene Expression. Lalwani MA, Zhao EM, Wegner SA, Avalos JL. ACS Synth Biol. 2021 Aug 20;10(8):2060-2075. doi: 10.1021/acssynbio.1c00229. Epub 2021 Aug 4. 10.1021/acssynbio.1c00229 PubMed 34346207
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