pET28a-Fgß-FIVAR-ELP-HIS-ybbR
(Plasmid #169136)

• Purpose: E. coli expression of FIVAR domain containing Fgß tag, ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.
• Depositing Lab: Michael Nash
• Publication: Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi: 10.1021/jacsau.2c00121. eCollection 2022 Jun 27.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5222
• Total vector size (bp): 6116
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: FIVAR (found in various architectures)
• Species: Clostridium perfringens
• Insert Size (bp): 894
• Tags / Fusion Proteins:
  • Fgβ peptide (N terminal on insert)
  • 3xELP linker (C terminal on insert)
  • 6x Histag (C terminal on insert)
  • ybbr tag (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • pET28a_Fg-FIVAR-ELP-HIS-ybbR.gb
• A portion of this plasmid was derived from a plasmid made by: This plasmid was derived from a pET28a vector encoding FIVAR (pET28a_ybbr-HIS-ddFLN4-8GS-FIVAR-Doc), which was a gift from Hermann Gaub and Lukas Milles at Ludwig-Maximilians-Universität Munich.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry