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pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR
(Plasmid #169135)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169135 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5222
  • Total vector size (bp) 6632
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Coh7
  • Alt name
    C7A
  • Species
    C. thermocellum CipA
  • Insert Size (bp)
    1410
  • Tags / Fusion Proteins
    • Fgβ peptide (N terminal on insert)
    • ddFLN4 tag (C terminal on insert)
    • 3xELP linker (C terminal on insert)
    • 6x Histag (C terminal on insert)
    • ybbr tag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR was a gift from Michael Nash (Addgene plasmid # 169135 ; http://n2t.net/addgene:169135 ; RRID:Addgene_169135)

  • For your References section:

    Optimal Sacrificial Domains in Mechanical Polyproteins: S. epidermidis Adhesins Are Tuned for Work Dissipation. Liu H, Liu Z, Yang B, Lopez Morales J, Nash MA. JACS Au. 2022 May 18;2(6):1417-1427. doi: 10.1021/jacsau.2c00121. eCollection 2022 Jun 27. 10.1021/jacsau.2c00121 PubMed 35783175
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