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His-SUMO- flag-hDDI2-D252N_pET11a
(Plasmid #169123)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169123 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-11a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hDDI
  • Species
    H. sapiens (human)
  • Mutation
    D252N
  • Entrez Gene
    DDI2

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-SUMO- flag-hDDI2-D252N_pET11a was a gift from Barbara Dirac-Svejstrup (Addgene plasmid # 169123 ; http://n2t.net/addgene:169123 ; RRID:Addgene_169123)

  • For your References section:

    DDI2 Is a Ubiquitin-Directed Endoprotease Responsible for Cleavage of Transcription Factor NRF1. Dirac-Svejstrup AB, Walker J, Faull P, Encheva V, Akimov V, Puglia M, Perkins D, Kumper S, Hunjan SS, Blagoev B, Snijders AP, Powell DJ, Svejstrup JQ. Mol Cell. 2020 Jul 16;79(2):332-341.e7. doi: 10.1016/j.molcel.2020.05.035. Epub 2020 Jun 9. 10.1016/j.molcel.2020.05.035 PubMed 32521225
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