pIB1884-ximD-PCPT
(Plasmid
#169109)
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PurposeProduction of marmesin in Streptomyces xiamenensis
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 169109 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSE152
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Apramycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameXimD and PcPT
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIB1884-ximD-PCPT was a gift from Minjuan Xu (Addgene plasmid # 169109 ; http://n2t.net/addgene:169109 ; RRID:Addgene_169109) -
For your References section:
Constructing Microbial Hosts for the Production of Benzoheterocyclic Derivatives. Bu XL, He BB, Weng JY, Jiang CC, Zhao YL, Li SM, Xu J, Xu MJ. ACS Synth Biol. 2020 Sep 18;9(9):2282-2290. doi: 10.1021/acssynbio.9b00405. Epub 2020 Aug 20. 10.1021/acssynbio.9b00405 PubMed 32786357