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Addgene

pET28a Gx-NL
(Plasmid #169105)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169105 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6043
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Bl21(DE3)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    protein G - NanoLuc
  • Species
    Synthetic
  • Insert Size (bp)
    810
  • Mutation
    Ala 24 stop
  • Promoter T7
  • Tags / Fusion Proteins
    • strep-tag (N terminal on insert)
    • his-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a Gx-NL was a gift from Maarten Merkx (Addgene plasmid # 169105 ; http://n2t.net/addgene:169105 ; RRID:Addgene_169105)

  • For your References section:

    Bioluminescent Antibodies through Photoconjugation of Protein G-Luciferase Fusion Proteins. Wouters SFA, Vugs WJP, Arts R, de Leeuw NM, Teeuwen RWH, Merkx M. Bioconjug Chem. 2020 Mar 18;31(3):656-662. doi: 10.1021/acs.bioconjchem.9b00804. Epub 2020 Jan 22. 10.1021/acs.bioconjchem.9b00804 PubMed 31909607
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