pAGM7547
(Plasmid
#169091)
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PurposeMoClo level 1 35S promoter-Db5GT-35S terminator transcription unit
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 169091 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepICH47831
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Backbone manufacturerSylvestre Marillonnet
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Vector typePlant Expression ; MoClo Cloning Vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name35S promoter-Db5GT-35S terminator
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Alt nameDb5GT
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SpeciesDorotheanthus bellidiformis
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAGM7547 was a gift from Sylvestre Marillonnet (Addgene plasmid # 169091 ; http://n2t.net/addgene:169091 ; RRID:Addgene_169091) -
For your References section:
Engineering Betalain Biosynthesis in Tomato for High Level Betanin Production in Fruits. Grutzner R, Schubert R, Horn C, Yang C, Vogt T, Marillonnet S. Front Plant Sci. 2021 Jun 9;12:682443. doi: 10.3389/fpls.2021.682443. eCollection 2021. 10.3389/fpls.2021.682443 PubMed 34177999