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pDRf1-SweetTrac1
(Plasmid #169083)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169083 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDRf1
  • Backbone size w/o insert (bp) 6939
  • Total vector size (bp) 8415
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SweetTrac1
  • Species
    A. thaliana (mustard weed), Synthetic
  • Insert Size (bp)
    1476
  • Promoter PMA1

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ACAGCACCAACAGATGTCGT
  • 3′ sequencing primer GAAGTGTCAACAACGTATCTACC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDRf1-SweetTrac1 was a gift from Lily S Cheung (Addgene plasmid # 169083 ; http://n2t.net/addgene:169083 ; RRID:Addgene_169083)

  • For your References section:

    Development and quantitative analysis of a biosensor based on the Arabidopsis SWEET1 sugar transporter. Park J, Chavez TM, Guistwhite JA, Gwon S, Frommer WB, Cheung LS. Proc Natl Acad Sci U S A. 2022 Jan 25;119(4). pii: 2119183119. doi: 10.1073/pnas.2119183119. 10.1073/pnas.2119183119 PubMed 35046045
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