pH6-spTorA-mCherry
(Plasmid #169040)

• Purpose: mCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.
• Depositing Lab: Siegfried Musser
• Publication: Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi: 10.1371/journal.pone.0256715. eCollection 2021.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 6117
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H6-spTorA-mCherry
• Species: Escherichia coli and Discosoma sp.
• Insert Size (bp): 864
• GenBank ID: CAD6011594.1 QIK02008.1
• Promoter: T7
• Tags / Fusion Proteins:
  • 6xHis (N terminal on insert)
  • spTorA (signal peptide of E. coli TorA) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7 Fwd
• 3′ sequencing primer: T7 Terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The spTorA-mCherry coding sequence was recovered from p-spTorA-mCherry-H6C, a plasmid first reported in Bageshwar et al., 2016 PLoS One, 11:e0149659.