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Addgene

pTorD-H6
(Plasmid #169039)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169039 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 5870
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TorD-H6
  • Species
    Escherichia coli
  • Insert Size (bp)
    624
  • Mutation
    The mutation N at position 46 was changed back to the wildtype S.
  • GenBank ID
    CAA52096.1
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 Fwd
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The TorD-6xHis coding sequence was recovered from pQE80TorADhis, a plasmid obtained from Tracy Palmer and first reported in Dow et al., 2013 Biochem. J., 452:57-66.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTorD-H6 was a gift from Siegfried Musser (Addgene plasmid # 169039 ; http://n2t.net/addgene:169039 ; RRID:Addgene_169039)

  • For your References section:

    Influence of the TorD signal peptide chaperone on Tat-dependent protein translocation. Bageshwar UK, DattaGupta A, Musser SM. PLoS One. 2021 Sep 9;16(9):e0256715. doi: 10.1371/journal.pone.0256715. eCollection 2021. 10.1371/journal.pone.0256715 PubMed 34499687
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