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pMCB320-sgZmat3.55.9
(Plasmid #169026)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169026 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMCB320
  • Backbone size w/o insert (bp) 8907
  • Vector type
    Mammalian Expression, Mouse Targeting, Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA targeting Zmat3, mCherry, Puromycin resistance
  • Alt name
    Zmat3, Wig1, PAG608
  • gRNA/shRNA sequence
    CTCCCCTGCCGTGGCAC
  • Species
    M. musculus (mouse)
  • Promoter mU6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstxI (unknown if destroyed)
  • 3′ cloning site Blp1 (unknown if destroyed)
  • 5′ sequencing primer GAG GCT TAA TGT GCG ATA AAA G
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Michael Bassik's lab, Stanford University

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCB320-sgZmat3.55.9 was a gift from Laura Attardi (Addgene plasmid # 169026 ; http://n2t.net/addgene:169026 ; RRID:Addgene_169026)

  • For your References section:

    Zmat3 Is a Key Splicing Regulator in the p53 Tumor Suppression Program. Bieging-Rolett KT, Kaiser AM, Morgens DW, Boutelle AM, Seoane JA, Van Nostrand EL, Zhu C, Houlihan SL, Mello SS, Yee BA, McClendon J, Pierce SE, Winters IP, Wang M, Connolly AJ, Lowe SW, Curtis C, Yeo GW, Winslow MM, Bassik MC, Attardi LD. Mol Cell. 2020 Nov 5;80(3):452-469.e9. doi: 10.1016/j.molcel.2020.10.022. 10.1016/j.molcel.2020.10.022 PubMed 33157015
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