Skip to main content
Addgene

pET15b p97-Strep-His E314Amb
(Plasmid #169021)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169021 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5783
  • Total vector size (bp) 8201
  • Modifications to backbone
    N-terminal His-tag removed, C-terminal SBP-His-tag added
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p97
  • Alt name
    vcp
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2418
  • Mutation
    E314TAG
  • GenBank ID
    NP_009057.1
  • Promoter T7
  • Tags / Fusion Proteins
    • SBP (C terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG
  • 3′ sequencing primer CTA GTT ATT GCT CAG CGG T
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b p97-Strep-His E314Amb was a gift from Hemmo Meyer (Addgene plasmid # 169021 ; http://n2t.net/addgene:169021 ; RRID:Addgene_169021)

  • For your References section:

    Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives PP1 Holoenzyme Formation. Weith M, Seiler J, van den Boom J, Kracht M, Hulsmann J, Primorac I, Del Pino Garcia J, Kaschani F, Kaiser M, Musacchio A, Bollen M, Meyer H. Mol Cell. 2018 Oct 11. pii: S1097-2765(18)30791-3. doi: 10.1016/j.molcel.2018.09.020. 10.1016/j.molcel.2018.09.020 PubMed 30344098
Related items: