pET15b His-p97
(Plasmid #169020)

• Purpose: Expresses His-p97 in bacteria
• Depositing Lab: Hemmo Meyer
• Publication: Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(18)30791-3. doi: 10.1016/j.molcel.2018.09.020.

Backbone

• Vector backbone: pET15b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5700
• Total vector size (bp): 8124
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p97
• Alt name: vcp
• Species: H. sapiens (human)
• Insert Size (bp): 2424
• GenBank ID: NP_009057.1
• Promoter: T7
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: TAA TAC GAC TCA CTA TAG GG
• 3′ sequencing primer: CTA GTT ATT GCT CAG CGG T

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry