pGEX6P1 UBXN11
(Plasmid
#169017)
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PurposeExpresses GST-UBXN11 (human) in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 169017 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX6P-1
- Backbone size w/o insert (bp) 4981
- Total vector size (bp) 6544
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUBXN11
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Alt nameUBXD5
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Alt nameSocius
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SpeciesH. sapiens (human)
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Insert Size (bp)1563
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GenBank IDNP_001376485.1
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Entrez GeneUBXN11 (a.k.a. COA-1, PP2243, SOC, SOCI, UBXD5)
- Promoter tac
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ATA GCA TGG CCT TTG CAG G
- 3′ sequencing primer GAG CTG CAT GTG TCA GAG G (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX6P1 UBXN11 was a gift from Hemmo Meyer (Addgene plasmid # 169017 ; http://n2t.net/addgene:169017 ; RRID:Addgene_169017) -
For your References section:
Protein Phosphatase-1 Complex Disassembly by p97 is Initiated through Multivalent Recognition of Catalytic and Regulatory Subunits by the p97 SEP-domain Adapters. Kracht M, van den Boom J, Seiler J, Kroning A, Kaschani F, Kaiser M, Meyer H. J Mol Biol. 2020 Nov 20;432(23):6061-6074. doi: 10.1016/j.jmb.2020.10.001. Epub 2020 Oct 12. 10.1016/j.jmb.2020.10.001 PubMed 33058883