pGEX6P1 p37
(Plasmid #169015)

• Purpose: Expresses GST-p37 (human, codon optimized for expression in bacteria)
• Depositing Lab: Hemmo Meyer
• Publication: Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-6074. doi: 10.1016/j.jmb.2020.10.001. Epub 2020 Oct 12.

Backbone

• Vector backbone: pGEX6P-1
• Backbone size w/o insert (bp): 4981
• Total vector size (bp): 5977
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p37
• Alt name: UBXN2B
• Species: H. sapiens (human)
• Insert Size (bp): 996
• Mutation: sequence was codon optimized for expression in E.coli
• GenBank ID: NP_001071087.1
• Entrez Gene: UBXN2B (a.k.a. p37)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: ATA GCA TGG CCT TTG CAG G
• 3′ sequencing primer: GAG CTG CAT GTG TCA GAG G

Resource Information

• Supplemental Documents:
  • pGEX6P1-p37 human (codon optimized).gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry