pProkR2.Cre.T2A.Venus
(Plasmid #169014)

• Purpose: Expresses Cre under Prok receptor 2 minimal promoter in mammalian cells
• Depositing Lab: Michael Hastings
• Publication: Prok2 Cre (unpublished)

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Backbone

• Vector backbone: N/A
• Backbone manufacturer: VectorBuilder
• Backbone size w/o insert (bp): 5282
• Total vector size (bp): 7124
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cre Recombinase
• Insert Size (bp): 1842
• Promoter: Prokineticin Receptor 2
• Tags / Fusion Proteins:
  • T2A
  • Venus

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: ctggccttttgctcacatgt

Resource Information

• Supplemental Documents:
  • pProkR2.Cre.T2A.Venus.WPRE.BGHpa (VB181016-1178faa).gb
• A portion of this plasmid was derived from a plasmid made by: The ProkR2 promoter sequence was determined by Antony Adamson (University of Manchester, UK) and the plasmid was constructed by VectorBuilder.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry