pCS2-LAMP1-mRFP
(Plasmid
#169004)
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PurposeExpress rat LAMP1-mRFP in zebrafish
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 169004 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2
- Backbone size w/o insert (bp) 3990
- Total vector size (bp) 5990
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerat LAMP1-mRFP
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1911
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Tag
/ Fusion Protein
- mRFP (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-LAMP1-mRFP was a gift from Noboru Mizushima (Addgene plasmid # 169004 ; http://n2t.net/addgene:169004 ; RRID:Addgene_169004) -
For your References section:
Autophagy Is Required for Maturation of Surfactant-Containing Lamellar Bodies in the Lung and Swim Bladder. Morishita H, Kanda Y, Kaizuka T, Chino H, Nakao K, Miki Y, Taketomi Y, Guan JL, Murakami M, Aiba A, Mizushima N. Cell Rep. 2020 Dec 8;33(10):108477. doi: 10.1016/j.celrep.2020.108477. 10.1016/j.celrep.2020.108477 PubMed 33296658