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Addgene

pCS2-ER-EmEGFP
(Plasmid #169000)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169000 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4200
  • Total vector size (bp) 4930
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL)
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    732
  • GenBank ID
    NM_004343.3
  • Entrez Gene
    CALR (a.k.a. CALR1, CRT, HEL-S-99n, RO, SSA, cC1qR)
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-ER-EmEGFP was a gift from Noboru Mizushima (Addgene plasmid # 169000 ; http://n2t.net/addgene:169000 ; RRID:Addgene_169000)

  • For your References section:

    Organelle degradation in the lens by PLAAT phospholipases. Morishita H, Eguchi T, Tsukamoto S, Sakamaki Y, Takahashi S, Saito C, Koyama-Honda I, Mizushima N. Nature. 2021 Apr;592(7855):634-638. doi: 10.1038/s41586-021-03439-w. Epub 2021 Apr 14. 10.1038/s41586-021-03439-w PubMed 33854238
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