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Addgene

ZP42 Vent-CS (ZV86)
(Plasmid #16900)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    zvox-1
  • Alt name
    vent
  • Alt name
    vega2
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    700
  • Entrez Gene
    vent (a.k.a. vega2, vent1, wu:fe36b11)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Zvox-1 in CS2+. EcoRI-HpaI fragment from Zvox-1-2 was inserted into KS EcoRI-Sma as ZV80. EcoRI-XbaI fragment from this was inserted into CS2+ EcoRI-XbaI.

To make RNA: cut with Asp718, transcribe with SP6.

Constructed by: David Kimelman, 2/1/97.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZP42 Vent-CS (ZV86) was a gift from Randall Moon (Addgene plasmid # 16900 ; http://n2t.net/addgene:16900 ; RRID:Addgene_16900)
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