ZP43 Wnt8 3' orf1
(Plasmid
#16899)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16899 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-T easy
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namewnt8 3' orf1
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Alt namewnt8
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)530
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Entrez Genewnt8a (a.k.a. wnt-8, wnt8, wnt8.1, wu:fa20e02, wu:fe05d07)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
530 bp PCR product ligated into vector pGEMTeasy. Purpose of clone is to provide an in situ probe specific for the first open reading frame of the bicistronic wnt8 RNA.
To make antisense probe: cut with Sph1, transcribe with SP6.
Citation: A. Lekven et al., 2001, Developmental Cell.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZP43 Wnt8 3' orf1 was a gift from Randall Moon (Addgene plasmid # 16899 ; http://n2t.net/addgene:16899 ; RRID:Addgene_16899) -
For your References section:
Zebrafish wnt8 encodes two wnt8 proteins on a bicistronic transcript and is required for mesoderm and neurectoderm patterning. Lekven AC, Thorpe CJ, Waxman JS, Moon RT. Dev Cell. 2001 Jul;1(1):103-14. doi: 10.1016/s1534-5807(01)00007-7. 10.1016/s1534-5807(01)00007-7 PubMed 11703928
Map uploaded by the depositor.