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Addgene

pYFAC
(Plasmid #168982)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168982 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKW20088
  • Backbone manufacturer
    doi:10.1038/nchembio.1366
  • Backbone size (bp) 14245
  • Vector type
    Synthetic Biology ; Heterologous expression in filamentous fungi.
  • Selectable markers
    URA3 ; Af pyrG

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For improved transformation efficiency, using a DH10B E coli such as 10-beta, and electroporation.
  • Copy number
    Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A detailed protocol on how to use this plasmid for fungal biosynthetic gene cluster elucidation projects can be found in Roux I., Chooi Y.H. (2022) Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans Using Episomal Vectors. In: Skellam E. (eds) Engineering Natural Product Biosynthesis. Methods in Molecular Biology, vol 2489. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2273-5_5

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYFAC was a gift from Yit Heng Chooi (Addgene plasmid # 168982 ; http://n2t.net/addgene:168982 ; RRID:Addgene_168982)

  • For your References section:

    Functional genomics-guided discovery of a light-activated phytotoxin in the wheat pathogen Parastagonospora nodorum via pathway activation. Chooi YH, Zhang G, Hu J, Muria-Gonzalez MJ, Tran PN, Pettitt A, Maier AG, Barrow RA, Solomon PS. Environ Microbiol. 2017 May;19(5):1975-1986. doi: 10.1111/1462-2920.13711. Epub 2017 Apr 10. 10.1111/1462-2920.13711 PubMed 28251756
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