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Addgene

ZP44 Wnt8 5' orf2
(Plasmid #16898)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16898 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    wnt8 5'orf2
  • Alt name
    wnt8
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    588
  • Entrez Gene
    wnt8a (a.k.a. wnt-8, wnt8, wnt8.1, wu:fa20e02, wu:fe05d07)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

588 bp PCR product ligated into BamHI and EcoRI cut pSK+.
For probe: cut with BamHI, use T7 RNA polymerase.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZP44 Wnt8 5' orf2 was a gift from Randall Moon (Addgene plasmid # 16898 ; http://n2t.net/addgene:16898 ; RRID:Addgene_16898)
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