pYFAC-CH2
(Plasmid #168978)

• Purpose: (Empty Backbone) AMA1 based expression in filamentous fungi pYFAC-pyrG carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
• Depositing Lab: Yit Heng Chooi
• Publication: Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. doi: 10.1039/c8sc02870b. eCollection 2019 Feb 7.

Backbone

• Vector backbone: pYFAC-pyrG
• Modifications to backbone: Contains four alcohol inducible promoters for heterologous expression: PalcA promoter, a bidirectional PalcS/M promoter and a PaldA along with terminators in between.
• Vector type: Synthetic Biology ; Heterologous expression in Aspergillus nidulans and related filamentous fungi
• Selectable markers: URA3 ; Af pyrG

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For improved transformation efficiency, using a DH10B E coli such as 10-beta, and electroporation.
• Copy number: Unknown

Resource Information

• Supplemental Documents:
  • pYFAC-CH2.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A detailed protocol on how to use this plasmid for fungal biosynthetic gene cluster elucidation projects can be found in Roux I., Chooi Y.H. (2022) Heterologous Expression of Fungal Biosynthetic Pathways in Aspergillus nidulans Using Episomal Vectors. In: Skellam E. (eds) Engineering Natural Product Biosynthesis. Methods in Molecular Biology, vol 2489. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2273-5_5