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Addgene

pYFAC-CH1
(Plasmid #168977)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 168977 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKW20088
  • Backbone manufacturer
    doi:10.1038/nchembio.1366
  • Backbone size (bp) 14245
  • Modifications to backbone
    PalcA promoter with PacI digestion site, CENS/ARS origin of replication
  • Vector type
    Synthetic Biology ; Heterologous expression in filamentous fungi.
  • Selectable markers
    URA3 ; Af pyrG

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For improved transformation efficiency, using a DH10B E coli such as 10-beta, and electroporation.
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYFAC-CH1 was a gift from Yit Heng Chooi (Addgene plasmid # 168977 ; http://n2t.net/addgene:168977 ; RRID:Addgene_168977)

  • For your References section:

    Heterologous biosynthesis of elsinochrome A sheds light on the formation of the photosensitive perylenequinone system. Hu J, Sarrami F, Li H, Zhang G, Stubbs KA, Lacey E, Stewart SG, Karton A, Piggott AM, Chooi YH. Chem Sci. 2018 Nov 22;10(5):1457-1465. doi: 10.1039/c8sc02870b. eCollection 2019 Feb 7. 10.1039/c8sc02870b PubMed 30809363
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