ZP46 Prickle-2
(Plasmid
#16896)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16896 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCS2P+
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePrickle-2
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SpeciesD. rerio (zebrafish)
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Entrez Geneprickle2b (a.k.a. cb886, im:7138215, pk2, prickle2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Partial length prickle-2, contains the entire protein except the last 75 amino acids (predicted). Cloned into the BamH1 and Cla1 sites of CS2P+.
To make antisense probe: cut with BamH1, transcribe with T7. For the sense strand cut with Cla1 and transcribe with SP6.
Citation: Michael T. Veeman et al., Current Biology 13: 680-685 (2003).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZP46 Prickle-2 was a gift from Randall Moon (Addgene plasmid # 16896 ; http://n2t.net/addgene:16896 ; RRID:Addgene_16896) -
For your References section:
Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. Veeman MT, Slusarski DC, Kaykas A, Louie SH, Moon RT. Curr Biol. 2003 Apr 15. 13(8):680-5. 10.1016/S0960-9822(03)00240-9 PubMed 12699626