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Addgene

ZP47 Nlk1
(Plasmid #16895)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16895 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEM-T
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nlk1
  • Alt name
    nlk
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    nlk1 (a.k.a. nlk, nlkb, zgc:111875)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For antisense probe cut with SacII and transcribe with SP6. For sense probe cut with Sal1 and transcribe with T7.

Citation: C. J. Thorpe and R. T. Moon (2004). Development 131:2899-2909.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZP47 Nlk1 was a gift from Randall Moon (Addgene plasmid # 16895 ; http://n2t.net/addgene:16895 ; RRID:Addgene_16895)
  • For your References section:

    nemo-like kinase is an essential co-activator of Wnt signaling during early zebrafish development. Thorpe CJ, Moon RT. Development. 2004 Jun;131(12):2899-909. doi: 10.1242/dev.01171. Epub 2004 May 19. 10.1242/dev.01171 PubMed 15151990