ZP47 Nlk1
(Plasmid
#16895)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16895 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEM-T
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNlk1
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Alt namenlk
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SpeciesD. rerio (zebrafish)
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Entrez Genenlk1 (a.k.a. nlk, nlkb, zgc:111875)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For antisense probe cut with SacII and transcribe with SP6. For sense probe cut with Sal1 and transcribe with T7.
Citation: C. J. Thorpe and R. T. Moon (2004). Development 131:2899-2909.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZP47 Nlk1 was a gift from Randall Moon (Addgene plasmid # 16895 ; http://n2t.net/addgene:16895 ; RRID:Addgene_16895) -
For your References section:
nemo-like kinase is an essential co-activator of Wnt signaling during early zebrafish development. Thorpe CJ, Moon RT. Development. 2004 Jun;131(12):2899-909. doi: 10.1242/dev.01171. Epub 2004 May 19. 10.1242/dev.01171 PubMed 15151990
Map uploaded by the depositor.