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pBluescript Sp5
(Plasmid #16884)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16884 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sp5
  • Alt name
    bts1
  • Species
    D. rerio (zebrafish)
  • GenBank ID
    AF388363
  • Entrez Gene
    sp5 (a.k.a. bts1, fc39b01, wu:fc39b01)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Zebrafish sp5 (bts1) cDNA Full-length in pBluescript SK, cloned into EcoRI / XhoI. Useful for making antisense probe. Is the paralog of sp5-like (sp5l, clone 27-i2 from the Wnt8 upregulated microarray screen). The clone comes from library screening (library from Bruce Appel) and the library was constructed using EcoRI and XhoI linkers; The library was made into the Uni-ZAP XR Lambda vector, and from it Bluescript SK was excised.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBluescript Sp5 was a gift from Laure Bally-Cuif (Addgene plasmid # 16884 ; http://n2t.net/addgene:16884 ; RRID:Addgene_16884)

  • For your References section:

    The zebrafish buttonhead-like factor Bts1 is an early regulator of pax2.1 expression during mid-hindbrain development. Tallafuss A, Wilm TP, Crozatier M, Pfeffer P, Wassef M, Bally-Cuif L. Development. 2001 Oct . 128(20):4021-34. PubMed 11641225
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