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Addgene

ZP59 23f17
(Plasmid #16883)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16883 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSPORT1
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    23f17
  • Alt name
    23f-17
  • Species
    D. rerio (zebrafish)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Clone 23f17 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library). Noncoding, repetitive transcript. Similar and overlapping with many other regions in the genome. For antisense probe synthesis.
Expressed in paraxial and ventral mesoderm during gastrulation. Later in specific brain regions.
For antisense: EcoRI, SP6.

Citation: G Weidinger and R. T. Moon (2005). Development.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZP59 23f17 was a gift from Randall Moon (Addgene plasmid # 16883 ; http://n2t.net/addgene:16883 ; RRID:Addgene_16883)

  • For your References section:

    The Sp1-related transcription factors sp5 and sp5-like act downstream of Wnt/beta-catenin signaling in mesoderm and neuroectoderm patterning. Weidinger G, Thorpe CJ, Wuennenberg-Stapleton K, Ngai J, Moon RT. Curr Biol. 2005 Mar 29. 15(6):489-500. 10.1016/j.cub.2005.01.041 PubMed 15797017
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