ZP60 Axin2
(Plasmid
#16882)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16882 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSPORT1
- Backbone size w/o insert (bp) 4100
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameaxin2
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SpeciesD. rerio (zebrafish)
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Entrez Geneaxin2 (a.k.a. axil, wu:fb37c08, zgc:111942)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Zebrafish axin2 cDNA (unclear if full-length or not) in pSPORT1. Clone 24h4 from microarray screen 1st round (Matthew Clark normalized late somitogenesis / 24 hpf / liver library). For antisense probe synthesis.
Pretty ubiquitously expressed during gastrulation. Positively regulated by Wnt signaling.
For antisense: Asp718, SP6.
Citation: G Weidinger and R. T. Moon (2005). Development.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ZP60 Axin2 was a gift from Randall Moon (Addgene plasmid # 16882 ; http://n2t.net/addgene:16882 ; RRID:Addgene_16882) -
For your References section:
The Sp1-related transcription factors sp5 and sp5-like act downstream of Wnt/beta-catenin signaling in mesoderm and neuroectoderm patterning. Weidinger G, Thorpe CJ, Wuennenberg-Stapleton K, Ngai J, Moon RT. Curr Biol. 2005 Mar 29. 15(6):489-500. 10.1016/j.cub.2005.01.041 PubMed 15797017