ZP62 Cystathionine beta-synthase
(Plasmid #16880)

• Depositing Lab: Randall Moon
• Publication: Weidinger et al Curr Biol. 2005 Mar 29. 15(6):489-500.

Backbone

• Vector backbone: pSPORT1
• Backbone size w/o insert (bp): 4100
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cystathionine beta-synthase
• Species: D. rerio (zebrafish)
• Entrez Gene: cbsb (a.k.a. cbs, cb442, wu:fb37g05, wu:fm61c07, wu:fq06c06, zgc:113704)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Zebrafish cystathionine beta-synthase cDNA (likely full-lenghth, at least contains full coding sequence) in pSPORT1. clone 6h9 from the microarray screen 1st round (Matthew clark normalized late somitogenesis / 24 hpf / liver library).
Expressed pretty ubiquitously early, but stronger in axail mesoderm during gastrulation. Later most prominently in eyes and somites.
For antisense: Asp718, SP6.

Citation: G. Weidinger et al Development 2005.