pAAV-GFAP-eLACCO1.1
(Plasmid #168790)

• Purpose: Astrocyte expression of fluorescent sensor for extracellular lactate
• Depositing Lab: Marie-Eve Paquet
• Publication: Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi: 10.1038/s41467-021-27332-2.

Backbone

• Vector backbone: pAAV
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eLacco1.1
• Insert Size (bp): 1821
• Promoter: GFAP
• Tags / Fusion Proteins:
  • GPI (C terminal on insert)
  • ER export (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: GFAP
• 3′ sequencing primer: WPRE

Resource Information

• Supplemental Documents:
  • pAAV_GFAP_eLACCO.gb
• A portion of this plasmid was derived from a plasmid made by: Robert Campbell

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.03.05.434048v1 for bioRxiv preprint.