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Addgene

XE1 Xwnt-3A
(Plasmid #16879)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16879 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64T
  • Backbone size w/o insert (bp) 3250
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xwnt-3A
  • Alt name
    wnt-3 A
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    3000
  • Entrez Gene
    wnt3a.L (a.k.a. XELAEV_18032766mg, Xwnt-3a, wnt-3a, wnt3a, wnt3a-A, xwnt3a)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Blunted EcoR1 into Bgl II (destroyed during cloning)
  • 3′ cloning site Blunted EcoR1 into Bgl II (destroyed during cloning)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: cDNA, 3 Kb.
Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by S. Wolda on the date of 5/22/92.

Notes for Use:
Citation: Wolda et al., Developmental Biology 155 (1993)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE1 Xwnt-3A was a gift from Randall Moon (Addgene plasmid # 16879 ; http://n2t.net/addgene:16879 ; RRID:Addgene_16879)

  • For your References section:

    Overlapping expression of Xwnt-3A and Xwnt-1 in neural tissue of Xenopus laevis embryos. Wolda SL, Moody CJ, Moon RT. Dev Biol. 1993 Jan. 155(1):46-57. 10.1006/dbio.1993.1005 PubMed 8416844
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