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Addgene

XE2 Xwnt-4
(Plasmid #16878)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16878 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64T
  • Backbone size w/o insert (bp) 2999
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xwnt-4
  • Alt name
    wnt4
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1000
  • Entrez Gene
    wnt4 (a.k.a. Xwnt4, wnt-4, xwnt-4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (unknown if destroyed)
  • 3′ cloning site Bgl II (unknown if destroyed)
  • 5′ sequencing primer Sp6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Transcription: linearize with Sal1 and transcribe with SP6.

Plasmid History:
Constructed by Lynn McGrew on date of 5/1/92.

Notes for Use: PCR & primers used to reconstruct AUG, TAG codons containing Bgl II sites to clone into pSP64T.

Early clone name: pX4.2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE2 Xwnt-4 was a gift from Randall Moon (Addgene plasmid # 16878 ; http://n2t.net/addgene:16878 ; RRID:Addgene_16878)
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