pRD393
(Plasmid
#168774)
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PurposeExpression of mEos3.2_H-NSdbd in Mycobacterium
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 168774 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFPV27
- Backbone size w/o insert (bp) 5625
- Total vector size (bp) 6480
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemEos3.2_H-NSdbd
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Alt namemEos3.2 HI-NESS
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SpeciesSynthetic
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Insert Size (bp)855
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer cgataccgtcgacctcg (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene plasmid #54550, Addgene plasmid #30178
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRD393 was a gift from Remus Dame (Addgene plasmid # 168774 ; http://n2t.net/addgene:168774 ; RRID:Addgene_168774) -
For your References section:
HI-NESS: a family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein. Rashid FM, Mahlandt E, van der Vaart M, Boer DEC, Varela Alvarez M, Henneman B, Brocken DJW, Voskamp P, Blok AJ, Shimizu TS, Meijer AH, Luijsterburg MS, Goedhart J, Cremazy FGE, Dame RT. Nucleic Acids Res. 2021 Nov 4. pii: 6414584. doi: 10.1093/nar/gkab993. 10.1093/nar/gkab993 PubMed 34734265