pMRX-IP-EGFP-mouse Plaat3 (N157stop)
(Plasmid #168771)

• Purpose: Expresses EGFP-Plaat3 (C-terminal element is deleted) in mammalian cells
• Depositing Lab: Noboru Mizushima
• Publication: Morishita et al Nature. 2021 Apr;592(7855):634-638. doi: 10.1038/s41586-021-03439-w. Epub 2021 Apr 14.

Backbone

• Vector backbone: pMRX-IPU-EGFP
• Backbone manufacturer: Dr.Shoji Yamaoka of Tokyo Medical and Dental University
• Backbone size w/o insert (bp): 6404
• Total vector size (bp): 7331
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hrasls3
• Alt name: Plaat3
• Alt name: Pla2g16
• Species: M. musculus (mouse)
• Insert Size (bp): 489
• GenBank ID: NM_001362425.1
• Entrez Gene: Plaat3 (a.k.a. AdPLA, HRSL3, Hrasls3, Hrev107, MLP-3, Pla2g16)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: It has the pMX backbone

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry