pB6-Id1-loxP-AU1-rox-FNF-loxP-drePBD*-rox
(Plasmid #168770)

• Purpose: B6J Id1 AU1 tag and LSL-TagRFP-T-drePBD* targeting vector, low copy number
• Depositing Lab: Mario Capecchi
• Publication: Miscellaneous gene targeting vector plasmids (unpublished)

Backbone

• Vector backbone: pWS-TK6
• Backbone manufacturer: Sen Wu, Capecchi laboratory
• Vector type: Mouse Targeting, Cre/Lox ; dre / rox
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Id1
• Species: M. musculus (mouse)
• Mutation: See Depositor Comments Below
• GenBank ID:
• Entrez Gene: Id1 (a.k.a. AI323524, D2Wsu140e, Idb1, bHLHb24)

Resource Information

• Supplemental Documents:
  • pId1-LSL-RFP-drePR.gb
• A portion of this plasmid was derived from a plasmid made by: FRT-Neo-FRT is from pFNF (#22687). TagRFP-T is from Roger Tsien. drePBD* is from Francis Stewart.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NGS sequencing during the QC process discovered mutations in the TagRFP-T-drePBD* sequence. Although these disrupt the drePBD* expression, this plasmid was deposited as is because the depositors had already generated a mouse line with the construct. The AU1 epitope tagged Id1 in that mouse line (with or without dre / rox recombination) should still be functional.

Homology arms subcloned by recombineering from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.

Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.