XE62 Xwnt-4 myc-SP64T
(Plasmid
#16877)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16877 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSP64T
- Backbone size w/o insert (bp) 2999
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameXwnt-4 myc
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Alt namexwnt4
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Alt namexwnt-4
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SpeciesX. laevis (frog)
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Insert Size (bp)1000
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Entrez Genewnt4 (a.k.a. Xwnt4, wnt-4, xwnt-4)
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Tag
/ Fusion Protein
- C-Myc (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See clone XE2 for details. A single c-myc epitope recognized by the monoclonal antibody 9E10 has been addes to the C-terminus.
Transcription: linearize with Sal1 and transcribe with SP6.
Citation: L. Lynn McGrew, unpublished.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE62 Xwnt-4 myc-SP64T was a gift from Randall Moon (Addgene plasmid # 16877 ; http://n2t.net/addgene:16877 ; RRID:Addgene_16877)