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Addgene

XE4 Xwnt-5A myc
(Plasmid #16874)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16874 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP64T
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Xenopus Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    full coding of Xwnt-5A
  • Alt name
    wnt5
  • Alt name
    xwnt
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1200
  • Entrez Gene
    wnt5a.L (a.k.a. Xwnt-5A, wnt-5a, wnt5a-a, wnt5a-b)
  • Tag / Fusion Protein
    • MYC

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert: full coding of Xwnt-5A.
Insert size: 1.2 Kb.
Transcription: linearize with EcoR1 and transcribe with SP6.
Plasmid History:
Constructed by RTM/JR on the date of 4/93.
Notes for Use:
This construct encodes full coding sequence of Xwnt-5A, without wnt UTR's.
See Xwnt 5A full-length for Genbank data.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    XE4 Xwnt-5A myc was a gift from Randall Moon (Addgene plasmid # 16874 ; http://n2t.net/addgene:16874 ; RRID:Addgene_16874)
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