XE174 Xenopus Wnt-5A-myc-pCDbeta-G
(Plasmid
#16873)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16873 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCD-betaG-PS
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Backbone manufacturerAvailable from Addgene (#17120)
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameWnt-5A
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Alt nameXwnt-5A
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SpeciesX. laevis (frog)
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Entrez Genewnt5a.S (a.k.a. XELAEV_18003033mg, wnt-5A, wnt5a, wnt5a-B, wnt5a-a)
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Tag
/ Fusion Protein
- myc
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The myc tagged Xwnt-5A insert of clone XE40, in the SV40 construct pCD-PS modified by Julia Yang-Snyder to include Xenopus beta globin flanking untranslated regions.
Reference: The clone was made by Julia Yang-Snyder.
Addgene QC found a G125A point mutation that is not known to affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
XE174 Xenopus Wnt-5A-myc-pCDbeta-G was a gift from Randall Moon (Addgene plasmid # 16873 ; http://n2t.net/addgene:16873 ; RRID:Addgene_16873)